5-Bromo-4-chloro-3-indolyl-B-D-galactoside
7240-90-6
408.6
C14H15BrCINO6
2938909090
-20°C
Chromogenic substrate of B-Galactosidase used in combination with IPTG for detection of B-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by B-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. After transformation the cells show B-Galactosidase activity in the presence of media containing X-GAL and IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of B-Galactosidase activity leading to the appearance of white colonies. Non-recombinant cells produce a blue indigo dye.
0.01 kg
3.20 x 4.40 x 3.20
Product usually ships within 24 hours.
Research or further manufacturing use only, not for food or drug use.
