Bacterial spot is an important bacterial disease of peppers. Two different bacteria, Xanthomonas campestris pv. vesicatoria (Xcv) and Xanthomonas vesicatoria (Xv) can incite this seed borne disease. mTMB (modified Tween Medium B) is a semi-selective medium for detection of Xcv and Xv on seeds of pepper and tomato. The colonies of Xcv and Xv on mTMB plates are yellow, slightly mucoid, mounded and round. Xcv utilizes Tween 80 and in 3-7 days a white crystalline halo usually forms around the yellow colony. Contaminated seed lots can be detected by dilution plating of the bacterial extract on CKTM, mKM or MXV. Suspected isolates are then transferred to YDC. Finally, the identity of the suspected isolates can be deter-mined by a pathogenicity test or PCR.
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