“Cellulase Onozuka R-10” from Trichoderma Viride.1 unit (U) of Cellulase will release 1.0 µmole of glucose from carboxymethyl cellulose. Routinely used for the isolation of protoplasts, for its ability to degrade cell walls. Cellulase “Onozuka R-10” is often used in combination with Macerozyme R-10 (cat. no. M 8002).Beldman, G. et al., The cellulase of Trichoderma Viride, . J. Biochem., 146, 301-308, 1985. Potrykus, J., et al., Protoplasts: Isolation, culture, plant regeneration, 118, 549-578, 1986. Tewes, A., et al., High yield isolation and rapid recovery of protoplasts from suspension cultures of tomato (Lycopersicon esculentum), 113, 141-150, 1984.Evans, D.A. et al., Plant protoplast isolation and culture, Int. Rev. Cyt. Suppl, 16, 33-53, 1983.
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