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X-GAL [5-Bromo-4-chloro-3-indolyl-B-D-galactoside], 100 Milligrams

Price£ 28.75
  • SKU: B71800-0.1
  • Pack Size: 100 MG
Low Stock

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X-GAL, 100 MG
X-GAL, 100 MG
X-GAL, 100 MG







Chromogenic substrate of B-Galactosidase used in combination with IPTG for detection of B-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by B-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. After transformation the cells show B-Galactosidase activity in the presence of media containing X-GAL and IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of B-Galactosidase activity leading to the appearance of white colonies. Non-recombinant cells produce a blue indigo dye.

0.01 kg

3.20 x 4.40 x 3.20

Product usually ships within 24 hours.
Research or further manufacturing use only, not for food or drug use.
  • Appearance: White powder
  • Identity (IR): Conforms
  • Purity (HPLC): ≥ 99.0 %
  • Solubility: Clear and colourless (2%, DMF)
  • Specific rotation(DMF:H2O 1:1 C=1): -63.0 - -60.0 °
  • Water Content : ≤ 1.0 %

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