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X-GAL [5-Bromo-4-chloro-3-indolyl-B-D-galactoside], 100 Milligrams

Price£ 8.25
  • SKU: B71800-0.1
  • Pack Size: 100 MG
Quantity
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Low Stock

Frequently Bought Together

X-GAL, 100 MG
X-GAL, 1 G
TRIS, Ultrapure, 1 KG
Total price: £ 68.75

This item: X-GAL, 100 MG £ 8.25

X-GAL, 1 G £ 26.00

TRIS, Ultrapure, 1 KG £ 34.50

X-GAL, 100 MG
X-GAL, 100 MG
X-GAL, 100 MG

5-Bromo-4-chloro-3-indolyl-B-D-galactoside

7240-90-6

408.6

C14H15BrCINO6

29389090

2-8°C


Chromogenic substrate of B-Galactosidase used in combination with IPTG for detection of B-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by B-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. After transformation the cells show B-Galactosidase activity in the presence of media containing X-GAL and IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of B-Galactosidase activity leading to the appearance of white colonies. Non-recombinant cells produce a blue indigo dye.


0.01 kg

3.20 x 4.40 x 3.20


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Research or further manufacturing use only, not for food or drug use.